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. 2023 Jul 25;6(8):1143–1154. doi: 10.1021/acsptsci.3c00046

Figure 2.

Figure 2

Fluorescent images and bar graphs showing the effects of JA and 22-(4′-py)-JA on NSCLC cell invasion. Following the transwell invasion assay, the invaded cells were identified by staining with DAPI and imaged by fluorescence microscopy. (A) 22-(4′-py)-JA (dose, ≥0.5 nM) decreased the number of invading H460 and A549 cells. (B) Same concentration of JA did not demonstrate any significant suppressive activity. Scale bar, 10 μm. (C) Cells were incubated with nontoxic doses of 22-(4′-py)-JA for 24 h, and MMP-2 and MMP-9 mRNA expressions were evaluated by qRT-PCR. (D) MMP-2 and MMP-9 activities assessed by gelatin zymography analysis revealed a marked reduction in the gelatinase activities of both enzymes. Data are presented as mean ± SEM (n = 3). *p < 0.05 vs untreated control group. All data were analyzed by using analysis of variance (ANOVA) followed by the Tukey post hoc test.