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. 2023 Jul 25;6(8):1143–1154. doi: 10.1021/acsptsci.3c00046

Figure 4.

Figure 4

Effects of of 22-(4′-py)-JA on the AKT/mTOR/p70S6K signaling pathway. Western blot images and bar graphs showing the levels of p-AKT, AKT, p-mTOR, mTOR, and p-p70S6K relative to the control in (A) H460 and (B) A549 cells treated with or without nontoxic concentrations of 22-(4′-py)-JA for 24 h. Western blot images and bar graphs showing the levels of p-AKT, AKT, p-mTOR, mTOR, and p-p70S6K relative to the control in (C) H460 and (D) A549 cells pretreated with either EGF (100 ng/mL) or LY294002 (10 μM) for 1 h and incubated with 1 nM 22-(4′-py)-JA for 24 h. The upregulation of p-AKTS473, p-mTOR S2448, and p-p70S6KT389 mediated by EGF was reversely declined in response to 22-(4′-py)-JA in both cell types. Data are expressed as mean ± SEM (n = 3). *p < 0.05 vs untreated control group. #p < 0.05 vs indicated group. All data were analyzed by using analysis of variance (ANOVA) followed by the Tukey post hoc test.