Fig. 6.
Inhibition of ERK prevents mPTP opening and protects from OGD/R injury by reducing Drp1 activation in SH-SY5Y cells. A The opening of mPTP was detected by loading with calcein AM (green fluorescence). The intensity of green fluorescence was negatively correlated with the opening of mPTP, scare bar: 100 μm. B Quantitative analysis of calcein AM florescence intensity. C, D, LDH and CCK-8 assays were performed to detect cell injury and viability. All data in this figure are representative of three independent repeats and are shown as mean ±SEM. si-NC: Drp1 si-RNA negative control group; si-Drp1: Drp1 si-RNA sequences; Drp1S616E: Drp1S616E-GFP-M90 plasmid; Vector: Drp1S616E negative control group. *P < 0.05 vs. Control, #P < 0.05 vs. Model, &P < 0.05 vs. si-NC, $P < 0.05 vs. Vector