Fig. 6.

Akt/GSK-3β signaling was activated to suppress the β-catenin degradation in CC with DM. a, b Total and phosphorylated Akt (Ser473) were evaluated by western blotting in cancerous and noncancerous adjacent tissues of CC with and without DM, respectively. c, d β-catenin, the phosphorylation levels of Akt (Ser473) and GSK-3β (Ser9) were detected by western blotting under HG and SNs conditions and after the treatment of Akt inhibitor MK-2206. The samples derive from the same experiment and that blots were processed in parallel. Data are means ± S.E.M. and analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001