Fig. 5.

Trisomy of Hsa21 does not alter cathepsin B maturation or lysosomal localisation. a Western blot showing pro-cathepsin B (pro-CatB) and mature cathepsin B (mat-CatB) protein levels in disomic and trisomy 21 human primary fibroblasts b pro-cathepsin B protein levels (Mann–Whitney U test p = 0.3429), c mature cathepsin B protein level (Mann–Whitney U test p = 0. 8857), d mature cathepsin B/pro-cathepsin B ratio (Mann–Whitney U test p = 0.6857) were not altered by trisomy 21. e, f Colocalisation of cathepsin B (cyan) with LAMP1 (magenta) was measured using immunofluorescence and no difference between disomic (Pearson’s Correlation R = 0.484) and trisomy 21 (Pearson’s Correlation R = 0.493) fibroblasts was observed (Mann–Whitney U test p = 0.6857). Individual data points are means of 3 technical replicates for n = 4 disomic and n = 4 trisomy 21 independent cell lines, error bars SEM