Table 2.

Bioinformatic search results for sequence identity with hCas9

Database	Program	Keyword limitation	Accession #	Organism	Description	Length (aa)	E value	% identity	Alignment length (aa)
NCBI-TRS	TBLASTN	Toxin	XM_040336796.1	Rana temporaria	Multidrug and toxin extrusion protein 1-like (LOC120926671), transcript variant X3, mRNA	1616	0.87	38	47
NCBI-TRS	TBLASTN	Toxin	XM_040336794.1	Rana temporaria	Multidrug and toxin extrusion protein 1-like (LOC120926671), transcript variant X1, mRNA	1740	0.89	38	47
NCBI-TRS	TBLASTN	Toxin	XM_040336795.1	Rana temporaria	Multidrug and toxin extrusion protein 1-like (LOC120926671), transcript variant X2, mRNA	1742	2.0	36	47
NCBI-EST	TBLASTN	Toxin	CMD69_G06_40	Sus scrofa	MNMPM3 Sus scrofa cDNA clone PPSUBLIB_56C06 5', mRNA sequence	594	10.0	42	38
BLAST UniProt-toxin	BLASTP	Toxin	A0A502HM27	Brevibacillus laterosporus (Bacillus laterosporus)	CRISPR-associated endonuclease Cas9	1092	2e−25	26	549
BLAST UniProt-toxin	BLASTP	Toxin	H1D476	Fusobacterium necrophorum subsp. funduliforme	Type II toxin-antitoxin system VapC family toxin	117	2e−18	43	119
BLAST UniProt-toxin	BLASTP	Toxin	A0A1G4RN64	Lachnospiraceae bacterium	Nucleotidyl transferase AbiEii/AbiGii toxin family protein	249	1.8	27	103
BLAST UniProt-toxin	BLASTP	Toxin	A0A1H2T7K0	Lachnospiraceae bacterium	Nucleotidyl transferase AbiEii/AbiGii toxin family protein	249	3.9	27	103
BLAST UniProt-toxin	BLASTP	Toxin	A0A2H0VRT0	Chlamydiae bacterium	Nucleotidyl transferase AbiEii/AbiGii toxin family protein	253	4.5	25	119
BLAST UniProt-toxin	BLASTP	Toxin	A0A7X8NY49	Citrobacter freundii	Insulinase family protein	497	0.47	22	168
BLAST UniProt-allergen	BLASTP	Allergen	A0A834R755	Sarcoptes scabiei	Mite allergen Der p 3	595	3.1	30	56
BLAST UniProt-allergen	BLASTP	Allergen	A0A3S2L044	Chilo suppressalis	Uncharacterized protein	194	6.9	45	31