Fig. 2.

Validation of a genetically engineered mouse with Tn+ feces. a) Breeding strategy to generate intestinal epithelial-specific Cosmc male KO, female mosaic, and WT mice. b) Western blot of male KO, female mosaic, and WT feces stained with ReBaGs6 (left), ReBaGs6 pre-blocked with Tn+ AsBSM (right). c) Biotinylated VVA lectin plus streptavidin-HRP (left), or streptavidin-HRP alone (right). d) Western blot with ReBaGs6 antibody (left) or PNA lectin (right) of WT and KO feces treated with T-synthase enzyme ± UDP-gal. Two independent repeats were performed for b)–d).