Fig. 4.

ATAM detection of Tn antigen in conditioned media. WT (Tn(−)) and Cosmc-deficient (Tn+) cell lines were cultured from ~20% to near confluency in media containing 10% FBS. Conditioned media at various dilutions (1:0 = no dilution to 1:2,000) was compared with fresh media (1:∞) by anti-Tn microarray. CRC (COLO-205; LS174T subclone), gastric cancer (MKN45), breast cancer (MCF7, MDA-MB-231), and kidney (HEK293) cell lines were used. Data were normalized to 100 μg/ml printed AsBSM for each well and then to max RFU across all cell lines. Two independent repeats were performed, error bars = ±1 SD.