TABLE 1.

Analysis of corrected LHSNO39 target loci in rescued plasmids

G418-resistant cellsa	Genomic DNA EcoRI digests on Southern blot (kb)b	Result for rescued plasmids
		EcoRI digest (kb)	Description or sequence of plasmidc
			5′ End of homology	neo gene	3′ End of homology	LTR-chromosome junctiond
Clone 1	NT	6.0	WT	WT	WT	TTTCATAGCTGGGAT
Clone 2	NT	5.0	WT	WT	WT	TTTCAAGACGAAAAA
Clone 3	9.0, 4.6	9.0	WT	WT	WT	TTTCACCCGGAGCCC
Clone 4	7.3, 4.2	4.2	WT	WT	WT	TTTCAGTACCATCTA
Clone 5	4.5	4.5	WT	WT	WT	TTTCAGCTTAAGGTA
Clone 6	6.7	6.7	WT	WT	WT	TTTCATGTAGAACAG
Clone 7	4.7	4.7	WT	WT	WT	TTTCACTTCCTGATC
Clone 8	5.0	5.0	WT	WT	WT	TTTCATCTCTTAAAA
Clone 9	NT	11	WT	WT	WT	TTTCAGCCTGCATGA
Clone 10	22, 5.5	5.5	Rearranged	WT	WT	TTTCATGCTGAACTA
Pool 1	Polyclonal	4.6	WT	WT	WT	TTTCAAAACCGCCCT
Pool 2	Polyclonal	13	WT	WT	WT	TTTCAATGCCACGCA

^aAll cells derived from MHF2 fibroblasts with LHSNO39 target sites corrected by AAV-SNO648. 

^bNT, not tested due to inadequate amount of genomic DNA. 

^cWT, wild type. 

^dThe first 5 nucleotides are from the LTR.