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Effect of dr1 mutations on Pr76 Gag expression. Total cellular protein was isolated from QT6 cells transfected with the indicated mutant DNAs isolated at 40 h posttransfection. Then, 30 μg of protein was loaded per lane and subjected to SDS-PAGE and Western transfer. Pr76 Gag was detected with rabbit antiserum reactive to p27 CA and p7 NC. An alkaline phosphatase-conjugated secondary antibody was used in conjunction with a fluorescent substrate to detect the bands.
