Figure 2.

The uni OPCs exhibit widespread distribution after being transplanted into CD (Nur7) mouse brains. A) The uni OPCs migrated and spread widely in the brain six months after transplantation. The serial sagittal dot maps of the human nuclear antigen (hNu) staining are shown. Each series was begun from the lateral to the midline and continued at 900 µm intervals. Scale bar: 2000 µm. B) The uni OPCs gave rise to mostly oligodendroglial lineage cells, a small population of astrocytes and few neurons in the transplanted mouse brains. Six months after transplantation, the uni OPC‐transplanted brains were immunostained for hNu and the oligodendroglial lineage markers OLIG2 and SOX10, the astrocyte marker SOX9 and the neuronal marker NeuN. Images from the corpus callosum (CC), the subcortical white matter (Sub), the brain stem (BS) and the cortex (Ctx) were shown. Scale bar: 50 µm. C) The total hNu⁺ human cells in whole brain from WT and uni OPC‐transplanted mice. n = 3 mice for WT and uni OPC‐transplanted mice. ns, not significant by Student's t‐test (two‐tailed). D) The percentage of the neural lineage marker⁺ cells in the transplanted hNu⁺ human cells. The hNu⁺ neural lineage marker⁺ cells in four regions of the brain, including the corpus callosum (CC), the subcortical white matter (Sub), the brain stem (BS) and the cortex (Ctx) were quantified. n = 6 fields from each region from 4 mice, with 2 images from each region in each mouse brain.