FIG. 2.
Effect of T22 on the chemotactic response of PHA-stimulated PBMC induced by SDF-1α. A total of 106 PHA- and IL-2-activated PBMC were washed with PBS(−) and suspended in RPMI 1640 containing 0.25% human albumin in the presence or absence of various concentrations of SDF-1α and T22 and 3 μM 4Ala-T-I. They were then added to the top chamber of a 5-μm (pore-size) polycarbonate Transwell culture insert and incubated for 30 min at 37°C. The bottom chamber contained 100 nM SDF-1α. The cells were cultured at 37°C for 3 h. The number of cells that transmigrated into the lower chamber was counted microscopically. The results are representative of three independent experiments.