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. 2023 Aug 15;6:850. doi: 10.1038/s42003-023-05224-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a UMAP (Uniform Manifold Approximation and Projection) non-linear dimension reduction is used to plot single cells (single dots) in relation to each other in 2D space. b Pseudotime trajectory plot showing predicted differentiation of germline cells (black line). The root node at which pseudotime is zero (indigo) was selected using known expression of the vasa gene (AGAP008578) in the premeiotic germline cells (black arrow). Pseudotime values are automatically assigned to remaining cells with higher values (yellow) representing a further differentiation from cells near the root node. c–l Linear expression levels of marker genes (counts per cell) for vasa (AGAP008578), Fas3 (AGAP029564), squid (AGAP000399), geminin (AGAP000496), RacGAP1 (AGAP008912), aubergine (AGAP011204), ß2-tubulin (AGAP008622), elf4E-5 (AGAP007172), protamine (AGAP028569) and Dnah3 (AGAP007675) are displayed on a graded colour scale.