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. 2023 Aug 15;6:850. doi: 10.1038/s42003-023-05224-z

Fig. 4. Total RNA content of Autosomal, X-linked and Y-linked genes.

Fig. 4

RNA content is calculated by taking the sum of all unique molecular identifiers (UMIs), which correspond to a single RNA molecule, that map to autosomal a, X chromosome b, or Y contigs c for each cell. Hub cells (HC), Germline stem cells (GSC), Primary spermatogonia (P-Spg), Late Spermatogonia/Primary spermatocytes (L- Spg/P-Spc), Spermatocytes (Spc), Late spermatocytes/Early spermatids (L-Spc/E-Spt), Late spermatids (L-Spt) and Mature spermatozoa (Spz). Statistical differences calculated using Wilcoxon tests with adjusted p-values using the Bonferroni correction for multiple testing (p.adj <0.0001 ***, p.adj <0.001 **, p.adj <0.05 *). The lower and upper quartiles are defined by the box with the median represented within. Whiskers show the maximum and minimum range of the data with any outliers beyond this displayed as circles.