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. 2023 Aug 15;14(8):520. doi: 10.1038/s41419-023-06049-8

Fig. 4. NSUN2 mediates NR_033928 m5C methylation and regulates its expression.

Fig. 4

AD M5C RIP analysis of NR_033928 methylation levels in GC cells transfected with si-NSUN2-1 or NSUN2 overexpressing vectors. E Sanger sequencing analysis of m5C sites within NR_033928. F, G RNA stability analysis of NR_033928 level in MKN45 cells transfected with si-NSUN2-1 or overexpressing NSUN2 vectors after treatment with 5 µg/mL actinomycin D. H, I RNA stability analysis of NR_033928 level in AGS cells transfected with si-NSUN2-1 or overexpressing NSUN2 vectors after treatment with 5 µg/mL actinomycin D. (Graph represents mean ± SD; **p < 0.01, and ***p < 0.001).