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. 2023 Aug 15;14(8):520. doi: 10.1038/s41419-023-06049-8

Fig. 5. NR_033928 promotes GC progression via GLS-mediated glutamine metabolism.

Fig. 5

A The volcano plot showing the dysregulated genes in NR_033928 knockdown MKN45 cells compared to wild type MKN45 cells. B The biological signaling pathways response upon NR_033928 knockdown by Gene KEGG analysis. C EDU assays in AGS cells transfected with NR_033928 overexpressing vectors with or without glutamine deletion. D Analysis of glutamate concentration in cells transfected with NR_033928 overexpressing vectors or co-transfected with sh-GLS lentivirus. E Apoptotic assays in AGS cells transfected with NR_033928 overexpressing vectors with or without glutamine deletion. F Analysis of α-KG concentration in cells transfected with NR_033928 overexpressing vectors or co-transfected with sh-GLS lentivirus. G EDU assays in AGS cells transfected with NR_033928 overexpressing vectors or co-transfected with sh-GLS lentivirus. H Apoptotic assays in AGS cells transfected with NR_033928 overexpressing vectors or co-treated with sh-GLS lentivirus. I EDU assays in AGS cells transfected with NR_033928 overexpressing vectors or co-treated with CB-839 (1 μm 72 h). J Apoptotic assays in AGS cells transfected with NR_033928 overexpressing vectors or co-treated with CB-839 (1 μm 72 h). (Graph represents mean ± SD; *p < 0.05, **p < 0.01 and ***p < 0.001).