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. 2023 Aug 15;14:4924. doi: 10.1038/s41467-023-40504-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Fiber photometry to record the calcium dynamics of LPB^Vglut2 neural terminals in the DMH with GCaMP6s. b Representative expression of GCaMP6s from LPB^Vglut2 neural terminals (left) and summary of fiber tracts (shown as blue dots, right) in the DMH with cold-induced cFos immunoactivity. This experiment was repeated at least 14 times independently with similar results. c A representative trace showing calcium dynamics of LPB^Vglut2 neural terminals in the DMH in response to warm and cold stimuli. d, e Calcium dynamics after warming or cooling the floor. (GCaMP6s, n = 14 mice; GFP, n = 5 mice). f–i Calcium dynamics (f, g) and quantification (h, i) of LPB^Vglut2 neural terminals in the DMH in response to different cool/cold floor temperatures. j–l Calcium dynamics (j) and quantification (k, l) of LPB^Vglut2 neural terminals in the DMH in response to cooling from different starting temperatures but the same temperature reduction. (n = 6 mice, 3–5 trials per mouse). m–o Calcium dynamics (m) and quantification (n, o) of LPB^Vglut2 neural terminals in the DMH in response to different cooling rates. (n = 10 mice, 2 trials per mouse). p, q Recording from DMH-projecting LPB^Vglut2 neurons (p) and representative expression of GCaMP6s (left) and summary of fiber tracts (shown as blue dots, right) (q). This experiment was repeated at least 6 times independently with similar results. r Calcium dynamics of DMH-projecting LPB^Vglut2 neurons in response to floor warming or cooling (n = 6 mice). s, t Peak ΔF/F₀ values (s) during floor warming or cooling and Mean ΔF/F₀ values (t) during the steady phase after floor warming or cooling (n = 6 mice). All data are the mean ± sem (except c), and (h, i, k, l, n, o) were analyzed by one-way RM ANOVA followed by Bonferroni’s multiple comparisons test, (e, s, t) were analyzed by unpaired t-test. P-values are calculated based on statistical tests in Supplementary Table 2. *p ≤ 0.05; **p ≤ 0.01; ns, not significant. Source data are provided as a Source data file.