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. 2023 Aug 15;14:4934. doi: 10.1038/s41467-023-40579-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Characterization of the BBTB permeability in PS5A1 GEMM using EZ-link biotin (Biotin, red, 660 Da) and Evans blue (EB, yellow, 66 kDa when bound to albumin) at 14-, 28-, and 42-days post injection (dpi). The tumor cells express GFP, and the cell nuclei are indicated by Hoechst staining (HOE, blue). The ROIs selected are (1) tumor core, (2) tumor margin, and (3) contralateral side with no tumor. The scale bars represent 1 mm in the top panel and 20 µm in the bottom panels. The blood vessels are indicated by arrows. b Characterization of the BBTB permeability in 73 C BBTB using EZ-link biotin (Biotin, red) and Evans blue (EB, yellow) at 7–21 dpi. The cell nuclei are indicated by Hoechst staining (HOE, blue). The ROIs selected are (1) tumor core, (2) tumor margin, and (3) contralateral side with no tumor. The blood vessels are indicated by arrows, and the dye leakage is indicated by asterisks. The scale bars represent 1 mm in the top panel and 20 µm in the middle and bottom panels. c, d The quantification of biotin and Evans blue coverage in PS5A1 and 73 C GEMMs by area fraction. Data are expressed as Mean ± SD. N = 15 images from 3 mice. Data in the box and whisker plots are given from the minima to maxima, the bounds of the box represent the 25th percentile and 75th percentile, and the middle line of the box is the median. Data were analyzed by One-way ANOVA followed by Tukey’s multiple comparisons test. n.s. represents no significant difference. Source data are available as a Source Data file.