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eIF4B in initiation complexes with PTB-depleted RRL. (A) Radioactivity profile of the gradient. The binding reaction with labeled IRES-912 RNA was performed as in Fig. 2, but PTB-depleted RRL was used instead of standard RRL. (B) UV-cross-linked proteins from the gradient shown in panel A. Molecular masses of marker proteins (M) are given in kilodaltons. 4B, eIF4B.
