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. Author manuscript; available in PMC: 2023 Aug 16.
Published in final edited form as: Methods Mol Biol. 2023;2631:155–182. doi: 10.1007/978-1-0716-2990-1_6

Figure 11: Set up and analysis of CRIS.py data for a large KI project.

Figure 11:

Example of the KI products obtained by Junction PCRs and the variables used in the CRIS.py program for a KI project using ssODN/ssDNA (a) or dsDNA (b). The PCR products shown in figures a and b can be referenced to the final PCR products in figures 4 and 5 respectively. The gene-specific portion of the primers is shown in purple and the DS tags in yellow. The variables in the script are shown as blue boxes. (c) Example of the .csv output file for 5junc PCR analysis by CRIS.py. (d) Example of the .csv output file for 3junc PCR analysis by CRIS.py.