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. Author manuscript; available in PMC: 2023 Aug 16.
Published in final edited form as: Methods Mol Biol. 2023;2631:155–182. doi: 10.1007/978-1-0716-2990-1_6

Figure 2: Basic Primer Design for small insertions and knockout modifications.

Figure 2:

Gene-specific forward and reverse primers (purple) with deep sequencing (DS) tags (yellow) are designed flanking the target modification and sgRNA site. The sgRNA site is the location of the 20-nucleotide protospacer and the protospacer adjacent motif (PAM).