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. Author manuscript; available in PMC: 2023 Aug 16.
Published in final edited form as: Methods Mol Biol. 2023;2631:155–182. doi: 10.1007/978-1-0716-2990-1_6

Figure 3: Primer Design and PCR strategy for large deletion projects.

Figure 3:

Gene-specific deletion primers (purple) with deep sequencing (DS) tags (yellow) are designed flanking the targeted deletion (red region flanked by sgRNA target sites (shown by green arrows). A second set of inside primers (pink) are designed inside the targeted deletion to determine if any untargeted or inverted alleles remain.