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. Author manuscript; available in PMC: 2023 Aug 16.
Published in final edited form as: Methods Mol Biol. 2023;2631:155–182. doi: 10.1007/978-1-0716-2990-1_6

Figure 4: Primer design and PCR strategy for large ssDNA KI projects.

Figure 4:

Two sets of primers are designed to the 5ʹ and 3ʹ regions of the targeted integration event. Each primer set consists of a gene-specific Gen primer (purple region in the Gen primer), which is outside the donor homology arms (shown in blue) and a Junc primer designed to a sequence in the unique sequence to be inserted (purple region in Junc primer). All primers contain DS tags (yellow).