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. Author manuscript; available in PMC: 2023 Aug 16.
Published in final edited form as: Methods Mol Biol. 2023;2631:155–182. doi: 10.1007/978-1-0716-2990-1_6

Table 2:

Reagents and reaction volumes for PCR#2

Reagent Volume (μL)
2× MyTaq or Superfi II 5
PCR2_Forward Primer (10μM) 0.5
dH20 2.5
Reverse index primer (10μM) 1
PCR #1 product 1
Total final reaction volume 10

Abbreviations: dH2O, nuclease-free water; PCR, polymerase chain reaction