FIG. 3.

Longitudinal analysis of vaccine-induced SIV-specific T cells. Groups of rhesus macaques were immunized with 8 μg of pTH.HW DNA with a gene gun or 5 × 10⁸ PFU of MVA.HW i.d. at the times indicated below each graph. Frozen isolated PBL samples were thawed, double stained with PE-conjugated tetrameric MHC-peptide complexes, and human CD8-specific Tri-color-conjugated MAb for the CD8 marker, and examined by FACS. An n indicates a sample measured, but negative.