FIGURE 1.

GSDMD activation in atherosclerotic lesion development in ApoE^−/− mice. ApoE^−/− mice were killed at the ages of 6, 8, 11, 14, and 18 weeks, and their aortas were harvested. (A) Representative aortic root cross-sections stained with H&E (scale bar = 200 μm). (B) Quantification of lesion area (n = 5). (C) Representative images of western blots. (D,E) Quantification of protein expression of p10 (caspase-1) and GSDMD-N by Western blotting (n = 4). β-actin served as the loading control. Data represent means ± SEM.