FIG. 7.

Filter binding assays used to measure the ability of T-ag to interact with the 31-bp site II oligonucleotide under replication conditions. The interaction of T-ag (0, 3, or 6 pmol) with 25 fmol of the 31-bp site II oligonucleotide was measured by nitrocellulose filter binding assays in the presence of the indicated nucleotide cofactors. The percentage of input oligonucleotide bound to a given filter was determined by scintillation counting. As a positive control, the interaction of T-ag (0, 3, or 6 pmol) with the 64-bp core oligonucleotide was measured in the presence of ATP. Additional controls were conducted with the 64-bp enhancer control and the 31-bp site IIm and the indicated nucleotide cofactors.