TABLE 2.
Viral replication in primary neuronal culturea
| HSV strain | Mean (SEM) viral output (PFU) |
|---|---|
| F | 1.0 × 107 (1.4 × 106) |
| hrR3 | 4.1 × 103 (4.2 × 102) |
| MGH1 | 1.4 × 103 (73) |
| Myb34.5 | 6.0 × 103 (4.3 × 102) |
| MybRevt | 1.5 × 103 (74) |
a
A total of 2.5 × 105 murine fetal (day 18) striatal neurons were harvested and plated as previously described (9). Two days after plating, cells were infected at an MOI of 0.1 with wild-type (strain F) and mutant viral strains in duplicate experiments. Twenty-four hours after infection, cells and supernatant were harvested and subjected to freeze-thaw cycles to liberate viral particles. Viral output was determined by plaque assay on Vero cells. Viral outputs of the four mutant viral strains (hrR3, MGH1, Myb34.5, and MybRevt) were not statistically different between groups (P > 0.3 [t test]).