TABLE 10.

Effect of CD8 cell depletion on protective responses stimulated by tl EDIM particles^a

Mouse strain	Rotavirus shedding (% antigen shed vs mock-immunized controls)
	Nondepleted	CD8 cell depleted
BALB/c	0.0	0.0
μMt	22.6	22.9

^aGroups of six (μMt) or eight (BALB/c) mice either were not inoculated or were inoculated daily with anti-CD8 monoclonal antibody (2.43) by using 1 mg/dose beginning 4 days prior to immunization. On the fifth day, mice were administered 10 μg of tl EDIM i.n., and anti-CD8 injections were continued twice a week for the duration of the study. Mice were administered a second i.n. dose of tl EDIM 2 weeks after the first dose, and 4 weeks later were challenged with EDIM. Shedding of rotavirus antigen was measured during the subsequent 7 days. The levels of CD8 cell depletion were measured on the day of immunization and 21 days after challenge and were found to be >98% in the mesenteric lymph nodes of both BALB/c and μMt mice.