FIG. 1.

Western analysis of p53 and MDM2 levels in NDFs. Proteins (150 μg) from one pool of NDFs (GM00011 cells) infected with virus carrying vector alone and from two pools of fibroblasts infected with virus expressing E7 were separated by polyacrylamide gel electrophoresis and blotted onto nitrocellulose. As a control for migration and immunoreactivity, human p53 protein was translated in a rabbit reticulocyte lysate. (A) p53 levels. Lane 1, lysate from NDFs carrying the vector alone; lanes 2 and 3, lysate from NDFs expressing E7; lane 4, 2 μl of a rabbit reticulocyte lysate programmed with RNA encoding p53; lane 5, 10 μl of a rabbit reticulocyte lysate programmed with RNA encoding p53. p53 was detected with monoclonal antibody DO-1. (B) MDM2 levels. Lanes 1 and 2, lysate from NDFs carrying the vector alone; lanes 3 and 4, lysate from NDFs expressing E7. MDM2 was detected with monoclonal antibody IF-2.