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Immunofluorescence detection reveals that p53 and MDM2 are in the nucleus in cells expressing E7. Pools of NDFs (GM00011) carrying the vector alone or expressing E7 were fixed and stained with p53 antibody DO-1 or MDM2 antibody 2A10. Antibodies were revealed with a secondary antibody conjugated to a fluorescent molecule (Alexa 488).
