FIG. 7.

Northern analysis of p21 mRNA in GM00011 cells expressing E7 alone, E7 and E6, or E7 and dominant-negative p53. First lane from left, NDFs carrying the vector alone; second lane, NDFs expressing E7; third lane, NDFs expressing E7 and dominant-negative p53; fourth lane, NDFs expressing E7 and E6. The blot was probed with a radiolabeled cDNA to p21 and reprobed with a radiolabeled cDNA to glyceraldehyde-3-phosphate dehydrogenase (GAPDH; a loading control). The fold increase in p21 mRNA was calculated as the ratio of the signal from p21 to the signal from GAPDH and normalized to signal from the vector control (n = 2).