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Live-dead assay to determine the viability of hiPSC-CM spheroids using C-AM and EthD-1. (a) Staining images of the spheroids grown on AggreWell™ 400 and PAMCELL™ R100 plate for C-AM (green), an indicator of live cells, and EthD-1 (red), an indicator of dead cells. Scale bar: 100 μm; (b) Verification of viability of hiPSC-CM spheroids through comparison of fluorescence (n = 50; * p ≤ 0.05); (c) Size of formed hiPSC-CM spheroids (n = 50) was measured by microscopy and analyzed by ImageJ
