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. Author manuscript; available in PMC: 2023 Aug 16.
Published in final edited form as: Gastroenterology. 2022 Feb 14;162(7):2004–2017.e2. doi: 10.1053/j.gastro.2022.02.017

Figure 6.

Figure 6.

CircANAPC7 inhibits ZIP4/miR-373–mediated muscle wasting in vitro and in vivo. (A) Relative body weight curve of mice injected with AsPC-shZIP4-Pre373-EV/circANAPC7 cells. (B) Mice body weight. (C) Grip strength plot of mice. (D) Representative images of tibialis anterior (TA) muscle and TA muscle weight relative to body weight. (E) Representative images of gastrocnemius (GAS) muscle and GAS muscle weight relative to body weight. (F) Representative images of H&E-stained TA muscle sections and quantitative cross-sectional area. (G) MyHC and Atrogin-1 protein levels were detected in GAS muscle tissues of mice. (H) UBR2, MuRF1, and atrogin-1 levels in C2C12 myotubes cultured in conditioned media for 8 hours. (I) MyHC levels in C2C12 myotubes cultured in conditioned media for 72 hours. (J, K) mRNA and protein levels of TGF-β in AsPC-shZIP4-Pre373-EV/circANAPC7 cells. (L, M) TGF-β levels in cell-conditioned media and tumor tissues were detected by enzyme-linked immunosorbent assay. (N) STAT5 and pSTAT5 levels were assessed by Western blot.