TABLE 1.

Infection with NL-r-HSAS virus derived from DAt1ruEGFP-transduced cells^a

Expt	% HSA in CEMx174 cells
	NL-r-HSAS infected				Mock infected, no vector
	DAt1ruEGFP	HR′CMVEGFP	SRαLEGFP	No vector
1	2.2	22	9.7	26	0.08
2	42	74	71	73	0.37

^aCell culture supernatants were harvested from cultures of vector-transduced CEMx174 cells infected with NL-r-HSAS at day 7 (MOI of 0.1) and at day 4 (MOI of 1) (shown in Fig. 2A). The p24 values of the supernatants from day 7 (MOI of 0.1) were 84.2 ng/ml for NL-r-HSAS-infected DAt1ruEGFP-transduced cells, 1,390 ng/ml for NL-r-HSAS-infected HR′CMVEGFP-transduced cells, 1,220 ng/ml for NL-r-HSAS-infected SRαLEGFP-transduced cells, 1,440 ng/ml for NL-r-HSAS-infected no-vector-transduced cells, and <0.08 ng/ml for mock-infected no-vector-transduced cells. The p24 values for supernatants from day 4 (MOI of 1) were 1,180 ng/ml for NL-r-HSAS-infected DAt1ruEGFP-transduced cells, 1,950 ng/ml for NL-r-HSAS-infected HR′CMVEGFP-transduced cells, 1,120 ng/ml for NL-r-HSAS-infected SRαLEGFP-transduced cells, 920 ng/ml for NL-r-HSAS-infected no-vector-transduced cells, and <0.08 ng/ml for mock-infected no-vector-transduced cells. Supernatants from day 7 (MOI of 0.1) were used for experiment 1. Supernatants from day 4 (MOI of 1) were used for experiment 2. Supernatants from day 7 (MOI of 0.1) were normalized by the p24 value (84.2 μg/ml) for infection. Fresh CEMx174 cells (5 × 10⁵) were infected for 2 h with 1 ml of each supernatant. Four days postinfection (experiment 1) or 7 days postinfection (experiment 2), cells (2 × 10⁵) were stained with a monoclonal antibody to HSA conjugated with PE, and 7 × 10³ events were analyzed for the percentage of HSA expression by flow cytometry.