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. 1999 Sep;73(9):7678–7693. doi: 10.1128/jvi.73.9.7678-7693.1999

FIG. 1.

FIG. 1

Kinetics of M84 protein expression and absence of virion association. NIH 3T3 cells were infected with K181 at an MOI of 3, and at 8, 24, and 48 h p.i., cells were harvested and whole-cell lysates were prepared as described in Materials and Methods. Cells were also treated for the first 8 h of infection with CHX before the 12-h-p.i. harvest or were infected and incubated in the presence of PAA until the 48-h-p.i. harvest. Western blots of lysates and purified virions (Vir.) were probed with an affinity-purified rabbit antiserum to GST-M84 or a GST-M83-specific antiserum. Each panel depicts the same blot, and lanes are numbered at the bottom. Lanes 8 to 10 depict lanes 5 to 7 after prolonged exposure, and lanes 11 to 13 show lanes 5 to 7 after the blot was stripped and reprobed with the M83 antiserum. Un., uninfected. Positions of molecular mass markers are shown on the left.