FIG. 11.

PCR amplification of MCMV DNA in latently infected BALB/c mice. (A) Control nested PCRs in which IE1-specific primer pairs were used to amplify serial dilutions of linearized HindIII L plasmid. Dilutions were made such that 25 to 0.2 copies of plasmid and 1 μg of uninfected mouse genomic DNA purified from the organs shown were amplified. The negative controls contained 1 μg of the organ DNA from uninfected mice, and PCR results for two to three individual uninfected mice are shown. All positive reactions produced only a single 310-bp product (shown) upon ethidium bromide staining, and representative results for the plasmid dilutions are shown (see Results). (B) Nested-PCR amplification of IE1 in the splenic stromal DNA isolated from four mice (no. 1 to 4) per group infected with 10⁵ PFU of tissue culture-derived K181, ΔM83, or ΔM84. Serial 10-fold dilutions of organ DNA were amplified independently. (C) Nested PCR of serial dilutions of salivary gland DNAs from mice 1 to 4 in each group as described for panel B. (D) Nested PCR of serial dilutions of lung DNAs from mice 1 to 4 as described for panel B.