FIG. 4.

Genomic analysis of ΔM83, ΔM83-2, and rΔM83 viruses by restriction digestion and Southern blotting. (Left panels) The restriction maps of the portions of the HindIII C regions under analysis are shown for the K181, ΔM83, and ΔM83-2 viruses, with arrows indicating the positions, lengths, and directions of transcription of ORFs. B, BamHI; St, StuI; X, XhoI. Above each restriction map are shown the lengths (in kilobase pairs) and positions of restriction fragments detected by the genomic probe (indicated by the shaded regions). (Right panels) Genomic DNA was purified from uninfected cells or cells infected with the virus indicated. Genomic and HindIII C plasmid DNAs were digested with BamHI, electrophoresed, and blotted. The 4.4-kbp StuI-to-XhoI genomic probe was isolated from the HindIII C plasmid, ³²P labeled by random priming, and used to probe the blot. The expected 0.52-kbp band in both panels was visible upon prolonged exposure of the blots.