Genomic analysis of the ΔM84 and rΔM83 viruses by restriction digestion and Southern blotting. Details of genomic maps and DNA preparation are as described in the legend to Fig. 4. Ss, SstI; RV, EcoRV; N, NotI; X, XhoI. Genomic and HindIII C plasmid DNAs were digested with SstI and XhoI prior to electrophoresis, blotting, and detection with the 7.6-kbp EcoRV-to-NotI genomic probe.