FIG. 4.

Nuclease S1 treatment of the products synthesized by NS5B by using the full-length HCV RNA template. RdRp-synthesized products were digested by nuclease S1 in 50 mM NaCl (low salt) or 500 mM NaCl (high salt). The Δ represents heat denaturation of the products at 96°C for 5 min and quick chilling on ice prior to the nuclease S1 treatment. Products were resolved on 1% agarose gel after heat denaturation of the samples at 96°C for 5 min. The arrowhead indicates the position of the RNA template.