FIG. 8.

In vitro binding of rainbow trout fibronectin and VHSV. Rainbow trout fibronectin (rt fibro) was purified from muscle by the gelatin affinity procedure, eluted, and extensively dialyzed. Purified fibronectin was mixed with VHSV (lane 4) or bovine rotavirus (lane 2) and pelleted through a glycerol cushion. Pellets were resuspended in Laemmli’s buffer, boiled, and analyzed on a Coomassie blue-stained SDS–10% polyacrylamide gel. Viral polypeptides are indicated on the left (rotavirus) and on the right (VHSV).