TABLE 2.

Blocking activity of the MAbs of various fish viruses^a

Viruses and cell line	Inhibition
Fish rhabdoviruses
PFR	+
SVC	+
C30	+
Perch rhabdovirus	+
VHSV	+
IHNV	+
Other fish viruses
IPNV	−
EHNV	−
OMV	−

^aAll viruses were grown in RTG-2 cells, and titers were calibrated in order to induce a total cytopathic effect 3 days postinfection. The inhibition was scored positive (+) when the cell monolayer was protected by MAbs from virus infection and negative (−) when cells were not protected. PFR, pike fry rhabdovirus; SVC, spring viremia of carp; C30, eel rhabdovirus; IPNV, infectious pancreatic necrosis virus (birnavirus); EHNV, epizootic hematopoietic necrosis virus (iridovirus); OMV, oncorhyncus masou virus (herpesvirus). All four serotypes of VHSV were used.