TABLE 3.
Relative abundance of VHSV cell target in various rainbow trout organs
| Organ(s)a | Immunoprecipitationb |
|---|---|
| Muscle | ++ |
| Heart | ++ |
| Front kidney | − |
| Rear kidney | − |
| Brain | + |
| Spleen | ± |
| Intestine | − |
| Gills | − |
a
A 0.2-g aliquot of each organ was washed, sliced, and lysed overnight in immunoprecipitation lysis buffer. Lysates were clarified, and the supernatants were subjected to immunoprecipitation.
b
The relative abundance of the immunoprecipitated product was visually estimated on Coomassie blue-stained SDS-polyacrylamide gels. ++, very abundant; +, fairly abundant; ±, hardly visible; −, not detectable.