FIG. 7.
Expression of BZLF1 mRNA 3 and 6 days after stimulation of CB193CR2 cell line (infected with EBV 70 days before) with TPA (5 × 10−8 M final concentration). RNA was extracted and submitted to PCR amplification with specific primers for BZLF1 mRNAs (the sequences are shown in Table 1). PCR amplification products were detected by staining with ethidium bromide (inverted picture). RT-PCR results from EBV-infected CB193CR2 cells without stimulation (lane 1), after 3 days of stimulation (lane 2), or after 6 days of stimulation (lane 3) are shown. M, molecular weight marker (100-bp ladder).