TABLE 1.
Sequences of primers used for RT-PCR and nested PCR and their positions in the genome of the EBV B95-8 strain
| Gene target | Oligonucleotide sequence (5′ to 3′) | Coordinates (nt) |
|---|---|---|
| EBER1 5′a | AAAACATGCGGACCACCAGC | 6776–6795 |
| EBER1 3′ | AGGACCTACGCTGCCCTAGA | 6648–6629 |
| EBNA1 5′RTb | CTTAGGAAGCGTTTCTTGAGCTT | 67482–67504 |
| EBNA1 3′RT | GGGTCTCCGGACACCATCTC | 108168–108149 |
| EBNA1 5′nested | GAGCGTTTGGGAGAGCTGAT | 67514–67533 |
| EBNA1 3′nested | CATTTCCAGGTCCTGTACCT | 107987–107967 |
| LMP2A 5′RTb | CACCGCTTATGAGGACCCA | 166729–166749 |
| LMP2A 3′RT | CTGCGGCCAGCAATGAAAC | 434–415 |
| LMP2A 5′nested | ATGACTCATCTCAACACATA | 166874–166893 |
| LMP2A 3′nested | CATGTTAGGCAAATTGCAAAA | 380–361 |
| LMP1 5′RTc | ACACACTGCCCTGAGGATGG | 169471–169490 |
| LMP1 3′RT | ATACCTAAGA/CAAGTAAGCA | 168956–168965 (or 169042–169051) |
| LMP1 5′nested | CTTCAGAAGAGACCTTCTCT | 169243–169262 |
| LMP1 3′nested | ACAATGCCTGTCCGTGCAAA | 169100–169081 |
| EBNA2 5′RTa | AGAGGAGGTGGTAAGCGGTCC | 14802–14822 |
| EBNA2 3′RT | TGACGGGTTTCCAAGACTATCC | 48584–48563 |
| EBNA2 5′nested | AGCGGTTCACCTTCAGGGCC | 14815–14832 (or 47761–47762) |
| EBNA2 3′nested | TGTAGGCATGATGGCGGCAG | 48512–48493 |
| BALF2 5′RT | GTCAAGATGTTCAAGGACGTGG | 163072–163094 |
| BALF2 3′RT | CTCATAGCACATACAGATGGGC | 162878–162856 |
| BALF2 5′nested | GGTCAAGAGCTGCTACCACG | 162978–162998 |
| BALF2 3′nested | GGAGATGTCCTGCAGGATGG | 162901–162881 |
| BZLF1 5′RTd | ATTGCACCTTGCCGGCCACCTTTG | 103180–103194 |
| BZLF1 3′RT | CGGCATTTTCTGGAAGCCACCCGA | 102486–102462 |
| BZLF1 5′nested | GACCAAGCTACCAGAG TCTAT | 103057–103077 |
| BZLF1 3′nested | CAGAATCGCATTCCTCCAGCCA | 102530–102512 (or 102657–102655) |
| BcLF1 5′RT | TATGCCCAATCCCAAGTACACG | 136210–136231 |
| BcLF1 3′RT | TGGACGGGTGGAGGAAGTCTTC | 135888–135867 |
| BcLF1 5′nested | ACACAGCAGCTACCGGTGGA | 136137–136176 |
| BcLF1 3′nested | TGTTGCAGGGAGTAGGTCTC | 136014–135995 |