. 2023 Apr 26;14(8):1492–1511. doi: 10.1039/d2md00371f

Effect of analogues 33, 40–42, 47 and 48 on purified dyn I lipid-stimulated GTPase activity (IC₅₀^a (μM)), in-cell endocytosis of Alexa labelled Tfn in U2OS cells (CME) or depolarisation-stimulated uptake of the styryl dye FM4-64 in synaptosomes (SVE).

Compound	Dynamin I IC₅₀^a (μM)	CME IC₅₀ (μM)	SVE IC₅₀ (μM)
33	3.7 ± 1.7	15.9 ± 1.6	264.9^b
40	3.9 ± 0.4	29 ± 4.3	126.8^b
41	4.4 ± 0.4	12.4 ± 4	Not active
42	3.6 ± 0.7	9.3 ± 2.5	Not active
47	3.5 ± 1.3	27.3 ± 2.0	12.3^c
48	3.0 ± 0.6	11.3 ± 2.4	Not active
49	5.2 ± 0.7	27.1 ± 0.8	Not active

IC₅₀ and 95% CI of triplicates, n = 1, except

and

. Mean and SEM of 3 and 2 independent experiments, each in triplicate respectively.

Effect of analogues 33, 40–42, 47 and 48 on purified dyn I lipid-stimulated GTPase activity (IC50a (μM)), in-cell endocytosis of Alexa labelled Tfn in U2OS cells (CME) or depolarisation-stimulated uptake of the styryl dye FM4-64 in synaptosomes (SVE).