TABLE 1.
Primers used for mutagenesis of pSwt and for recombinant genome sequencing
| Primer | Sequence (5′ to 3′)a | MHV-A59 genome location (nucleotide no.)b |
|---|---|---|
| FIJ-79 | GCGAATTATAGTGGTGGCACAC | 944–966 (HE ORF) |
| JJ2 | cgcgaagcttgaatccTAGGGTATA TTGGTGATTTTAGATGTATCC | 36–73 (S gene) |
| RS221 | GTTTATCCGCATAGTACGCAT | 661–641 (S gene) |
| A59-4 | TCAACTTGCCTACTTCGGGG | 1205–1186 (S gene) |
| WZL-15 | AGCAAAAGCCCAGATAGA | 3114–3131 (S gene) |
| JJ3 | gcggatccaagtCCtGcAGgGGCTG TGATAGTCAATCCTCATGAGA | 3994–3961 (S gene) |
a
Restriction sites introduced into primers are shown in lowercase letters.
b
Reverse numbering indicates a negative-strand primer. HE ORF, hemagglutin esterase open reading frame.