FIG. 4.

Specific coimmunoprecipitation of NS2 and full-length CRM1 proteins from MVM-infected FREJ4 cell extracts. Lysates of ³⁵S-labeled FREJ4 cells, infected (i.) or not infected (n.i.) with MVM at an MOI of 10 PFU per cell, were prepared in the presence (+) or absence (−) of SDS and immunoprecipitated with antisera directed against either the NS2 C terminus (SP6) (A and B), the NS1 C terminus (SP8) (A), or the hCRM1 C terminus (anti-hCRM1) (B). The autoradiograms show immunoprecipitated materials after separation by electrophoresis through 10% (A) or bipartite 6 to 10% (B) SDS-polyacrylamide gels. Arrows to the left point at phosphorylated (p) and nonphosphorylated (np) NS2 (25 kDa), 14-3-3 (30 and 32 kDa), NS1 (83 kDa), and CRM1 (110 kDa) proteins. M, molecular sizes (in kilodaltons) of prestained protein standards.