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Gating strategy analyzing viability of EpCAM positive organoids and EpCAM negative fibroblasts
A single cell suspension was stained with EpCAM – bv421, AnnexinV – APC and Propidium Iodide. Debris was removed by their scatter properties (FSC x SSC) and EpCAM positive (organoids) and EpCAM negative (fibroblasts) were analyzed on the Annexin V – APC versus PI scatter for live (bottom left), early (bottom right) and late apoptosis (upper right).
