Fig. 4.

Biological properties and stability of phage phiPA1-3. (A) Adsorption assays of phiPA1-3 with host PA001. (B) One-step growth curve of phiPA1-3 in the culture broth of PA001. (C) Lysis kinetics of phiPA1-3 on host PA001, at MOIs of 0.01, 0.1, 1, 10, and 100. Phages were incubated for 1 h under different temperatures (D) and pH levels (E), after which the stability of phiPA1-3 was determined in terms of plaque-formation on host lawns. Changes in PFUs were calculated relative to the control. The results are represented as mean ± standard deviation of triplicate experiments. MOI, multiplicity of infection.